mutL [2019-02-19 20:12:36]

sequence-unspecific endonuclease, DNA mismatch repair

locus: BSU17050

pI: 5.58

mw: 70.25 kDa

protein length: 627 aa

gene length: 1884 bp

function: DNA repair

product: DNA mismatch repair protein, sequence-unspecific endonuclease

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.7|Mismatch repair (MMR)]

Gene

Coordinates: 1,778,337 1,780,220

Phenotypes of a mutant

• 60-fold increased mutation rate [Pubmed|23882084]
• a ''[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB] [gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]'' mutant is not viable [Pubmed|23882084]

The protein

Protein family

• DNA mismatch repair mutL/hexB family (according to Swiss-Prot)

Structure

• [PDB|3KDK] (C-terminal domain) [Pubmed|20603082]

[SW|Localization]

• forms foci at midcell position, the frequency of foci increases upon mismatch formation [Pubmed|21958350]

Expression and Regulation

Operons

• genes: [gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]
  description: [Pubmed|8760914]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|mutL]' and '[protein|search|ymzD]' [PubMed|20525796]

Biological materials

Mutant

• GP1190 ([gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]::''aphA3'') [Pubmed|22178973], available in [SW|Jrg Stlke]'s lab
• 1A833 ( ''mutL''::''spec''), [Pubmed|11779496], available at [http://bgsc.org/search.php?Search=1A833 Bacillus Genetic Stock Center]
• BKE17050 ([gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTGCCACATTCATCACCC, downstream forward: _UP4_TAGCGGGGGTGGTAGGCATT
• BKK17050 ([gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTGCCACATTCATCACCC, downstream forward: _UP4_TAGCGGGGGTGGTAGGCATT

References

Reviews

• 22933559,26354434,26343983

Original publications

• 8760914,15375129,20603082,20525796,22843852,21050827,21958350,22178973,23882084,23998896,25326311,26384423,26163658,27369079,27851738,30391220